Figure 2: Representative bisulfite multiplex resequencing results of 50% methylated control sample using different modified primers. | Scientific Reports

Figure 2: Representative bisulfite multiplex resequencing results of 50% methylated control sample using different modified primers.

From: Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite-PCR sequencing

Figure 2

Sequencing results of representative libraries were prepared and sequenced using custom multiplex bisulfite resequencing. (a) Comparison of methylation profile of a representative amplicon (left) where a single CpG in the forward primer is substituted with one of the four modified bases), and CpG-free control (right), where amplicons were generated using CpG-free control primers produced a consistent level of methylation between the former and later indicating no prejudice in the multiplex reaction. (b) The portion of amplicons generated from primers with each modified base is within a similar range to the control amplicons used in each pool. (c) Modified bases corresponding to the box and whiskers plot in (b).

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