Figure 4: Representative result of amplicons generated using primers with either single or double CpG during bisulfite multiplex PCR. | Scientific Reports

Figure 4: Representative result of amplicons generated using primers with either single or double CpG during bisulfite multiplex PCR.

From: Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite-PCR sequencing

Figure 4

(a) Methylation profile of representative regions amplified with primers with a single CpG (a CpG is included in the forward primer), Double CpG (a CpG is included in both the forward and reverse primer), and CpG-free (no CpG) Control. When the C’s of the relevant CpGs were substituted with either degenerate or deoxyinosine bases, there was a similar level of methylation between the modified primers (primers with modified bases) and the CpG-free control. (b) The proportion of amplicons (of 75% methylated control) generated from primers pairs with either a single CpG or double CpG, and CpG-free controls compared using a box and whiskers plot.

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