Figure 5: Representative result of amplicons generated using different conditions. | Scientific Reports

Figure 5: Representative result of amplicons generated using different conditions.

From: Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite-PCR sequencing

Figure 5

The proportion of amplicons (of 50% methylated control) generated from assays with ‘no modifications’, ‘single modifications’ or double modifications. Baseline level of methylation (50%) is highlighted as ★. The number of amplicons in each assay and the corresponding primer concentration and melting temperature is as summarised in table. The [primer concentration] represents the concentration of the forward and reverse primer for every PCR assay in the multiplex pool.

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