Table 2 Summary of single-plex bisulfite PCR results where the modified base is located near the centre or 5′ end of the forward primer.
Forward Primer | Reverse Primer | Position of modification | Deoxyinosine | Mixed base | 5-nitroindole | Dspacer | CpG-free control |
|---|---|---|---|---|---|---|---|
C571 | C572 | None | — | — | — | — | Positive |
C575 | C576 | None | — | — | — | — | Positive |
C601 | C602 | Centre | Positive | Positive | No amplification | No amplification | — |
C603 | C604 | Centre | Positive | Positive | No amplification | No amplification | — |
C605 | C606 | Centre | Positive$ | Positive$ | No amplification | No amplification | — |
C607 | C608 | Centre | Positive | Positive | No amplification | No amplification | — |
C609 | C610 | Centre | Positive$ | Positive$ | No amplification | No amplification | — |
C611 | C612 | 5′ | Positive | Positive | No amplification | No amplification | — |
C613 | C614 | 5′ | Positive | Positive | Positive | Positive | — |
C615 | C616 | 5’ | Positive | Positive | Positive | Positive | — |
C617 | C618 | 5′ | Positive | Positive | No amplification | No amplification | — |
C619 | C620 | 5′ | Positive | Positive | No amplification | No amplification | — |
