Figure 7: DEVDase enzyme activity.

DEVDase activity after addition of algicide IRI-160AA expressed as relative fluorescence units per hour per μg of protein. Activity was measured 18 (P. minimum) or 24 (K. veneficum and G. instriatum) hours after addition of the algicide and normalized to total protein content. Bars represent triplicate means of the DEVDase activity in the presence (light gray) and absence (dark gray) of the Ac-DEVD-CHO inhibitor. Error bars represent one standard deviation of the mean (n = 3). An X indicates little to no DEVDase activity in controls. Significant differences between controls and treatments for each species are next to the species name in the margins while significant differences between + and − inhibitor are above the light gray bars (+inhibitor). In both cases significance is indicated by asterisks: *p < 0.05; **p < 0.01; ***p < 0.001. The inset figure represents algicidal activity accompanying the DEVDase experiment. For inset: error bars represent one standard deviation of the mean (n = 3).