Figure 1: ABLs carrying selected bioactive lipids facilitate phagolysosome maturation. | Scientific Reports

Figure 1: ABLs carrying selected bioactive lipids facilitate phagolysosome maturation.

From: Liposomes loaded with bioactive lipids enhance antibacterial innate immunity irrespective of drug resistance

Figure 1

dTHP1 cells were uninfected (a) or infected (b) with BCG at the MOI of 5, treated with ABLs carrying the indicated bioactive lipid for 18 hours and then stained with Lysosensor green DND189 for pH determination. Results are shown as mean ± standard deviation of the values obtained from the triplicate of each condition and are representative of three separate experiments. *p < 0.001 in comparison with untreated control by one sided Student’s t Test. (c) BCG was labeled with 100 μg/ml of the pH sensitive dye NHS and used to infect dTHP1 cells (MOI 5). Infected cells were then treated with ABLs carrying the indicated bioactive lipid. The fluorescence expressed in relative fluorescence units is directly proportional to intraphagosomal pH. Results are shown as mean ± standard deviation of the values obtained from the triplicate of each condition and are representative of three separate experiments. *p < 0.001 in comparison with untreated control by one sided Student’s t test.

Back to article page