Figure 4: Demixing profile of the Reaction.

In the graph is shown the profile of demixing of the reaction at different gradients of temperature. Temperature induces a reduction of the process time from 24 h to 6.5–7 h. Best results are obtained performing an Isothermal at 35 °C for 5.5 h and a ramp from 35 °C to 23 °C for 1.5 h. In details, after the isothermal step, the reaction of coacervation occurs, and a demixing of the prepared solution is observed. After the demixing phase, a variation of the temperature should be suddenly performed to avoid aggregation phenomena and the destruction of the nanovectors. Indeed, as soon as the demixing phase occurs, the produced nanoparticles are unstable and a further support of temperature needed to increase the stability of the system.