Figure 4: Confocal imaging of TRPM8, NFκB and TNFα expression in PC12 cells.
From: TRPM8 in the negative regulation of TNFα expression during cold stress
(A) The expression of TRPM8, NFκB and TNFα in wild type cells and Trpm8 knockdown cells at 37 °C and 4 °C. KD signifies Trpm8 knockdown cells. (B) In wild type cells, TRPM8 was upregulated and NFκB and TNFα were downregulated under cold conditions. (C) In KD cells, TRPM8 showed weak expression and NFκB and TNFα expression levels were increased. (D) Co-localization of TRPM8 and TNFα in the cytoplasm (cold condition and 500 nM menthol). Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01.
