Table 1 Oligonucleotide sequences for real-time RT-PCR.
Oligonucleotide Name | Sequence 5′-3' |
|---|---|
DENV gene oligonucleotide sequences | |
Type 1- 5′ NS5 | 5′-CAGGTCAAACGCAGCTATTG |
Type 1- 3′ NS5 | 5′-CCACTCCACTGAGTGAATTC |
Type 2- 5′ NS5 | 5′-TGTATGCCGATGACACCGCA |
Type 2- 3′ NS5 | 5′-TCTTTGCACACGGACCACCT |
Type 3- 5′ NS5 | 5′-TCAGAACTAACGCAGCCATG |
Type 3- 5′ NS5 | 5′-AGAGTTTTCACGCGAGAACC |
Type 4- 5′ NS5 | 5′-AGATCAAACGCAGCCATAGG |
Type 4- 5′ NS5 | 5′-CTTCCACTCCACTCCATGAA |
Human gene oligonucleotide sequences | |
5′ GAPDH | 5′-GTCTTCACCACCATGGAGAA |
3′ GAPDH | 5′-ATGGCATGGACTGTGGTCAT |
5′ OAS1 | 5′-CAAGCTTAAGAGCCTCATCC |
3′ OAS1 | 5′-TGGGCTGTGTTGAAATGTGT |
5′ OAS2 | 5′-ACAGCTGAAAGCCTTTTGGA |
3′ OAS2 | 5′-GCATTAAAGGCAGGA AGCAC |
5′ OAS3 | 5′-CACTGACATCCCAGACGATG |
3′ OAS3 | 5′-GATCAGGCTCTTCAGCTTGG |
5′ PKR | 5′-ATGATGGAAAGCGAACAAGG |
3′ PKR | 5′-GAGATGATGCCATCCCGTAG |
5′ IFN-alpha 2 | 5′-GCA AGT CAA GCT GCT CTG TG |
3′ IFN-alpha 2 | 5′-GAT GGT TTC AGC CTT TTG GA |
5′ IFN-alpha 5 | 5′-AGTTTGATGGCAACCAGTTC |
3′ IFN-alpha 5 | 5′-TCAGAGGAGTGTCTTCCACT |
5′ IFN-alpha 17 | 5′-AGG AGT TTG ATG GCA ACC AG |
3′ IFN-alpha 17 | 5′-CAT CAG GGG AGT CTC TTC CA |
Mice gene oligonucleotide sequences | |
5′ GAPDH | 5′-CCATGCCATCACTGCCACCC |
3′ GAPDH | 5′-GCCATGCCAGTGAGCTTCCC |
5′ OAS1 | 5′-GGACCCCGCTGACCCAACAA |
3′ OAS1 | 5′-CAACCTCCGTCGGCACCTCC |
5′ OAS2 | 5′-GGTGGGTCTTCAGGGGTGCC |
3′ OAS2 | 5′-GCAGCAGGTCCCAGATGGCA |
5′ OAS3 | 5′-CCGTGCCTGGACTGAGCCTC |
3′ OAS3 | 5′-GGCTGAGGTTTGGTGCCGGA |
5′ PKR | 5′-AGAGCCCGCCGAAAACTGCC |
3′ PKR | 5′-CGCTGTTAAACCTGGCGTCCA |
5′ IFN-alpha 2 | 5′-TGACCTCCACCAGCAGCTCA |
3′ IFN-alpha 2 | 5′-TCTGCTCTGACCACCTCCCA |
5′ IFN-alpha 5 | 5′-CCATCCCTGTCCTGAGTGAGCT |
3′ IFN-alpha 5 | 5′-AGATTCCTGCACCCCGACCT |
