Figure 1: Temporal and spatial expression patterns of OPN protein in 3-NP-treated rat forebrain. | Scientific Reports

Figure 1: Temporal and spatial expression patterns of OPN protein in 3-NP-treated rat forebrain.

From: Spatiotemporal expression of osteopontin in the striatum of rats subjected to the mitochondrial toxin 3-nitropropionic acid correlates with microcalcification

Figure 1

(a) Low-magnification views of representative coronal sections from saline-treated controls and rats subjected to 3-NP treatment. No basal expression of OPN is detected in the cortex or striatum of saline-treated controls or the cortex of rats injected with 3-NP. However, there is a well-demarcated induction of OPN immunoreactivity in the lateral part of the striatum. Note that OPN expression appears to be preferentially localised to the periphery of the lesion 14 days post-injury. AC, anterior commissure; CC, corpus callosum; LV, lateral ventricle. (b) Immunoblotting for OPN using protein extracts from microdissected cortex (C) and striatum (S) of saline-treated controls and 3-NP-treated rats 3, 7, and 14 days post-lesion. OPN protein is barely detectable in the cortex and striatum of the controls and the cortex of the experimental rats, while one major band of 70 kDa and two minor bands of 50 and 25 kDa are clearly induced in striatal tissue on days 3 and 7 post-lesion. Fourteen days after 3-NP treatment, two OPN-reactive bands of 70 and 50 kDa are still observed, although they have weaker intensity. At this time, the 25 kDa band is no longer detected.

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