Figure 3: Chevron plot analysis of UCH-L5N240. | Scientific Reports

Figure 3: Chevron plot analysis of UCH-L5N240.

From: Entropic stabilization of a deubiquitinase provides conformational plasticity and slow unfolding kinetics beneficial for functioning on the proteasome

Figure 3

(A) The observed reaction rates of the four kinetic phases derived from SJ stopped-flow fluorescence measurements shown in filled symbols. For the two slowest kinetic phases (black and red), some of the unfolding rates were derived from far-UV CD through manual mixing (open symbols). For the two faster kinetics phases, DJ interrupted refolding-based reaction rates are shown in open symbols. The results are fit to a two-state folding model for each kinetic phase and the fitting results are shown in solid lines. (B) Amplitudes associated with the four kinetic phases derived from SJ stopped-flow fluorescence measurements. The color-coding are the same as that in panel A. (C) Comparison of the slowest kinetic phases of UCH-L1 (red), -L3 (green) and UCH-L5N240 (blue). Extrapolations of the unfolding rates as a function urea concentration are shown in dashed line.

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