Figure 1: The Ct values of RNA (RSV) or DNA (ADV) obtained from the duplex RT-qPCR based on the assessment of different influencing factors. | Scientific Reports

Figure 1: The Ct values of RNA (RSV) or DNA (ADV) obtained from the duplex RT-qPCR based on the assessment of different influencing factors.

From: Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections

Figure 1

For GTC concentrations (A) for RSV, there were no statistical differences among groupA2, A3 and A4, but there were statistical differences among group A2, A3, A4 and group A1 (F = 51.29, P = 0.00); for ADV, the differences among all the groups weren’t statistically significant (F = 1.94, P = 0.20). For DTT concentrations (B) for RSV, there were no statistical differences among group B3, B4, B5 and B6, but there were statistical differences among group (B3, B4, B5, B6), group B2 and group B1 (F = 38.84, P = 0.00); for ADV, there were no statistical differences among group B3, B4, B5, B6, but there were statistical differences among group (B3, B4, B5, B6), group B2, group B1 (F = 10.18, P = 0.00). For magnetic bead amounts (C) for RSV, there were no statistical differences among group C2, C3 and C4, but there were statistical differences among group C2, C3, C4 and group C1 (F = 185.32, P = 0.00); for ADV, there were no statistical differences among group C2, C3 and C4, but there were statistical differences among group C2, C3, C4 and group C1 (F = 42.11, P = 0.00). For temperatures (D) for RSV, there were no statistical differences among group D2, D3 and D4, but there were statistical differences between group D2, D3, D4 and group D1 (F = 44.56, P = 0.00); for ADV, there were no statistical differences among group D2, D3 and D4, but there were statistical differences between group D2, D3, D4 and group D1 (F = 77.52, P = 0.00). For pH (E) for RSV, there was no statistical differences between group E5 and E6, and so was between group E3 and E4, but there were statistical differences among group (E5, E6), group (E3, E4), group E1 and group E2 (F = 85.36, P = 0.00); for ADV, there was no statistical differences between group E5 and E6, and so was between group E3 and E4, but there were statistical differences among group (E5, E6), group (E3, E4), group E1 and group E2 (F = 1370.60, P = 0.00).

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