Figure 2: The comparison of Ct values obtained from the different nucleic acid extraction methods.

F1: RNA carrier A; F2:RNA carrier B; F3: no RNA carrier; F4: TIANGEN; F5: TaKaRa RNA/DNA extraction kits that extract RNA and DNA separately. For different viral nucleic acid extraction methods, For Ct values of RSV (RNA), there were statistical differences among all the groups through SNK test (F = 106.73, P = 0.00). For Ct values of ADV (DNA), the differences among groups F5, F4 and F1 were not statistically significant, but there were statistical differences among group F2, F3 and group (F1, F4, F5) (F = 75.14, P = 0.00).