Table 1 Optimal quantities and equivalent Ct values for both virus of viral nucleic acid extraction.
Parameters/Assays | Value | RSV Ct | ADV Ct |
---|---|---|---|
GTC concentrations (M) | 2 | 20.35 ± 0.30 | 25.07 ± 0.20 |
DTT concentrations (mM) | 80 | 20.78 ± 0.14 | 25.52 ± 0.16 |
magnetic beads (μl) | 20 | 20.31 ± 0.20 | 25.29 ± 0.15 |
Incubation temperature (°C) | 80 | 20.30 ± 0.28 | 25.59 ± 0.12 |
pH | 8 or 9 | 19.71 ± 0.55 or 20.33 ± 0.72 | 25.66 ± 0.14 or 25.47 ± 0.30 |
RNA carriers A (μl) | 6 μl | 16.80 ± 0.30 | 22.67 ± 0.57 |