Table 1 Optimal quantities and equivalent Ct values for both virus of viral nucleic acid extraction.

From: Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections

Parameters/Assays

Value

RSV Ct

ADV Ct

GTC concentrations (M)

2

20.35 ± 0.30

25.07 ± 0.20

DTT concentrations (mM)

80

20.78 ± 0.14

25.52 ± 0.16

magnetic beads (μl)

20

20.31 ± 0.20

25.29 ± 0.15

Incubation temperature (°C)

80

20.30 ± 0.28

25.59 ± 0.12

pH

8 or 9

19.71 ± 0.55 or 20.33 ± 0.72

25.66 ± 0.14 or 25.47 ± 0.30

RNA carriers A (μl)

6 μl

16.80 ± 0.30

22.67 ± 0.57

  1. It is average Ct values that are presented.