Figure 6: Establishment of a model of osteoporosis rats.

(A–D) Micro-CT showed that OVX-treated rats had significantly less trabecular bone formation compared with the sham-operated group (n = 6). (E,F) Compared with the sham group, H&E staining of the tibia 3 months post-OVX showed significantly sparser trabecular bone in OVX rats (n = 6). (G–K) At 3 months after surgery, BMD, BV/TV, Tb.N, Tb.Th and Conn.D decreased relative to sham rats, and Tb.Sp increased (n = 6). Statistical analyses were done using Students’t-test. *p < 0.05 was considered significant.