Figure 6: The C terminus of hTRIR contains key RNase domain.
From: Identification of an RNase that preferentially cleaves A/G nucleotides
(A) Truncated plasmids of hTRIR as diagrammed. (B) Purified truncated proteins. Plasmids diagrammed in A were transformed to BL21 and the expressed protein was purified. Proteins were separated by 15% SDS-PAGE and stained with Commassie blue. (C) RNase digestion assay. Truncated hTRIR proteins were incubated with T7 transcribed RNA and signals were detected by Typhoon Cyclone. (D) Statistical analysis of digestion efficiency of different hTRIR mutants in (C). Experiments were repeated at least three times and student t test were performed. Values of p < 0.05 were considered as statistically significant and marked with *.
