Figure 7: hTRIR cleaves RNA from 5′ end or 3′ end.
From: Identification of an RNase that preferentially cleaves A/G nucleotides
(A) Chemically synthesized RNA oligos with methylated group at either 5′ end or 3′ end were shown. (B) and (C) In vitro RNA digestion assay. RNA oligos with methylated group at 5′ end or 3′ end were incubated with hTRIR and separated by 7 M urea 20% PAGE. RNA was stained with stain-all and scanned with Epson scanner. (D) and (E) In vitro RNA digestion assay. RNA oligos with methylated group at 5′ end or 3′ end were incubated with Sen15 and separated by 7 M urea 20% PAGE. RNA was stained with stain-all and scanned with Epson scanner. (F) The cartoon of potential mechanism of hTRIR in RNA digestion.
