Figure 2: Change of genetic quasispecies is essential for adaptation to new target cells.
From: Quasispecies of Hepatitis C Virus Participate in Cell-Specific Infectivity
(A) Infectious titers in the supernatants on serial passages of Hep3B/miR-122 and Huh7.5.1 cells infected with HCVcc/Huh7 (left panel) and HCVcc/Hep3B (right panel). (B) Infectious titers in the supernatants on serial passages of FU97 cells infected with HCVcc/Huh7 (red line) and HCVcc/Hep3B (blue line). (C) Distribution of mutations through the adaptation to Huh7.5.1 and/or Hep3B/miR-122 cells. Huh7- and/or Hep3B-adapted HCVcc were obtained through serial passages. Sequences were determined by direct sequencing and compared with JFH1-WT. (D) Frequency and position of mutations in HCVcc/Huh7 and HCVcc/Hep3B through serial passages in Huh7.5.1 cells. Produced viral particles in the supernatants were purified by ultracentrifugation, and total RNA was applied to deep sequence analysis. Frequencies of mutations in HCVcc/Huh7 and HCVcc/Hep3B were listed at every passage, and were classified into 4 patterns (am1-am4). (E) Changes in the frequencies of adaptive mutations are shown in the line graph.
