Figure 2: Binding affinity of hnRNP A2 for the LTR-II + III + IV G4 and mutant sequences measured by surface plasmon resonance (SPR) analysis. | Scientific Reports

Figure 2: Binding affinity of hnRNP A2 for the LTR-II + III + IV G4 and mutant sequences measured by surface plasmon resonance (SPR) analysis.

From: The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes

Figure 2

The human recombinant hnRNP A2 was immobilized on the SPR chip by amine coupling chemistry and the unlabelled oligonucleotides were bound at a flow rate of 25 μl/min, with contact time and dissociation time of 120 s in HEPES-KCl buffer. Sensograms were obtained in the oligonucleotide concentration range of 31.25 nM–2 μM. SPR sensograms of (a) LTR-II + III + IV with ka 4.7 × 104 ± 5.2 × 102 Ms−1 and kd 9.2 × 10−4 ± 7.5 × 10−5 s−1, (b) LTR-II + III + IV M4-5 with ka 2.8 × 104 ± 3.4 × 102 Ms−1 and kd 10.0 × 10−4 ± 8.4 × 10−5 s−1 and (c) LTR-II + III + IV Random, the ka and kd values of which are not reliably measurable. Sensograms are shown as grey lines and their respective fits as black lines.

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