Figure 3: Taq polymerase stop assay in the presence of hnRNP A2. | Scientific Reports

Figure 3: Taq polymerase stop assay in the presence of hnRNP A2.

From: The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes

Figure 3

(a) Taq polymerization was performed in the presence/absence of K+ and hnRNP A2, as indicated, on the wt and the mutant LTR-II + III + IV M4 + 5 sequences. Amplification of the wt template was performed at 37 °C for 30 min. Stop regions corresponding to LTR-III are indicated by the * symbol, to LTR-II by § and to LTR-IV by#. M is a marker lane obtained with the Maxam & Gilbert sequencing protocol. P indicates the band of the labelled primer. (b) Quantification of lanes 1–3 in panel (a). Quantification of stop bands corresponding to formation of LTR-II and LTR-III G4s and of the full-length amplification product (FL) is shown.

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