Figure 4: FRET analysis of hnRNP A2 unfolding.

(a) FRET-melting curves of LTR F-II + III + IV-T, in the absence or presence of hnRNP A2 1:10 or of BSA as control. (b) Corresponding first derivative curves, dF/dT versus T. Proteins were incubated with the LTR G4s for 30 min at 37 °C prior to analysis. (c) Unfolding % of all tested sequences at 100, 50, 25 mM K⁺, incubated in the presence of hnRNP A2 or BSA at 37 °C for 30 min. Unfolding values are referred to unfolding in the absence of proteins (100%).