Table 1 MS analysis of protein content of three independent experiments and collected from SDS gels.

From: The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes

Exp#

Experimental monoisotopic mass

Expected monoisotopic mass

Protein match

Peptide match

Score*

1

1921.102

1921.079

Putative zinc finger protein 812

V145-R162

18

1,2, 3

2188.904 2219.053

2188.898 2219.063

Heterogeneous nuclear ribonucleoprotein A2/B1

N326-R350 D130-R147

27 18

1

2429.146

2429.157

Heterogeneous nuclear ribonucleoprotein D0

I198-K218

31

2

1681.900

1681.907

Heterogeneous nuclear ribonucleoprotein C1/C2

M74-K89

39

2

863.487

863.487

Mediator of RNA polymerase II transcription subunit 11

V97-K103

22

2

1036.698

1036.676

Histone H1.2

A192-K201

20

2

1036.698

1036.687

Transcription factor AP-4

R294-R302

18

3

952.346

952.349

Cysteine/serine-rich nuclear protein 2

D249-R257

16

3

3450.685

3450.699

Non-POU domain-containing octamer-binding protein

G399-R434

28

  1. *The score (assigned on probabilistic bases by mascot software, “ http://www.matrixscience.com/help/interpretation_help.html#THRESHOLDS”)51 is the probability that the observed match is not a random event. The score is reported as −10x log10(P) where P is the absolute probability. The mass of the putative peptides and of the 50 most intense fragment ions were used for the data base search. Significant Mascot hits were accepted as positive matches and their ion score reported. The highest score obtained in the three experiments is reported.
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