Table 2 Melting temperatures (Tm) of LTR G4s measured on dual-labelled oligonucleotides by FRET and on unlabelled oligonucleotides by CD.

From: The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes

LTR

K+ (mM)

Tm (°C)

FRET

CD

II + III + IV

100

49.1 ± 0.1

61.1 ± 0.2

50

45.1 ± 0.1

nd

25

43.2 ± 0.1

nd

III + IV

100

55.2 ± 0.1

64.6 ± 0.1

50

49.1 ± 0.1

nd

25

46.2 ± 0.1

nd

III

100

62.1 ± 0.1

68.7 ± 0.5

50

56.0 ± 0.1

nd

25

52.1 ± 0.1

nd

IV

100

59.1 ± 0.1

50.6 ± 0.8

50

57.1 ± 0.1

nd

25

51.1 ± 0.1

nd

  1. Tm values are reported with standard deviation. Nd stands for “not-determined”.
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