Figure 1: Analysis of Recombinant Human HAT-L4. | Scientific Reports

Figure 1: Analysis of Recombinant Human HAT-L4.

From: The Transmembrane Serine Protease HAT-like 4 Is Important for Epidermal Barrier Function to Prevent Body Fluid Loss

Figure 1

(A) HAT-L4 protein domains. The transmembrane (TM), SEA and protease domains and active sites His (H), Asp (D) and Ser (S) are indicated. (B) A purified HAT-L4 fragment, consisting of the protease domain, was analyzed by SDS-PAGE and Coomassie blue staining. (C) A full-length recombinant human HAT-L4 containing a C-terminal V5 tag was expressed in CHO cells and analyzed by Western blotting using anti-V5 (left panel) and anti-HAT-L4 (right panel) antibodies. Vector-transfected CHO cells were used as controls. (D) Flow cytometric analysis of HAT-L4-expressing (lower panels) and vector-transfected (upper panels) CHO cells using anti-V5 (left panels) and anti-HAT-L4 (right panels) antibodies.

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