Figure 4: Disruption of the mouse Tmprss11f gene. | Scientific Reports

Figure 4: Disruption of the mouse Tmprss11f gene.

From: The Transmembrane Serine Protease HAT-like 4 Is Important for Epidermal Barrier Function to Prevent Body Fluid Loss

Figure 4

(A) Illustration of CRISPR/Cas9-based targeting strategy to delete a 20-bp sequence in Tmprss11f exon 4. The WT (Tmprss11f+) allele (upper), the targeted (Tmprss11f−) allele (lower) and PCR primers (black arrowheads) for genotyping are indicated. (B) Representative PCR genotyping results of Tmprss11f+/+, Tmprss11f+/− and Tmprss11f−/− mice. PCR fragments were analyzed in ethidium bromide-stained agarose gel. (C) DNA sequencing traces confirming Tmprss11f− (upper) and Tmprss11f−/− (lower) alleles. The targeted nucleotide sequence in the Tmprss11f+/+ allele (upper, boxed) was deleted in the Tmprss11f−/− allele (lower). (D) RT-PCR analysis of HAT-L4 mRNA expression in tongues from Tmprss11f+/+ and Tmprss11f−/− mice. A negative control without cDNA templates in RT-PCR (no cDNA) was included in the experiment. Beta-actin mRNA expression was used as a positive control.

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