Figure 3: Soluble hVEGFR3 gene therapy did not affect plaque burden and plaque-associated lymphangiogenesis, yet induced an increase in plaque T cell content.
From: Adventitial lymphatic capillary expansion impacts on plaque T cell accumulation in atherosclerosis
(A) Western blot showing cropped image of blot and (B) ELISA analysis of hVEGFR3 expression in plasma after AAV-hVEGFR3-Ig gene transfer. (C) Lymph vessel formation and ingrowth in an in-vivo matrigel plug assay, four weeks after s.c. administration of AAV-hVEGFR3-Ig gene transfer. Quantification of (D) plaque volume, (E) plaque classification and (F) routine pathological examination of plaque composition of lesion formation in carotid arteries. (G) Quantification of adventitial and plaque T cell content. (H) Percentage of Lyve-1+ lymphatic capillary area in the adventitia.
