Figure 5: 53BP1 affects parkin-mediated mitophagy.
From: 53BP1 contributes to regulation of autophagic clearance of mitochondria
Control and 53BP1-deficient U2OS cells (A) and 53BP1 WT and 53BP1 KO H1299 cells (B) were treated with CCCP for the indicated time points. Total cell extracts and mitochondrial fractions were analyzed by Western blotting with antibodies against 53BP1, Parkin, LC3-II, p62, COX-1, and β-actin as the control. (C) 53BP1 stably knockdown U2OS cells were transiently transfected with either the HA-tagged 53BP1 shRNA resistant vector or a control vector (HA-Control). Twenty-four hours after transfection, cells were treated with CCCP for 6 h. Total cell extracts and mitochondrial fractions were then analyzed using the indicated antibodies.
