Figure 4: Histological evaluation of colon inflammatory cell infiltration in female and male BTBR mice. | Scientific Reports

Figure 4: Histological evaluation of colon inflammatory cell infiltration in female and male BTBR mice.

From: Sex-related alterations of gut microbiota composition in the BTBR mouse model of autism spectrum disorder

Figure 4

Representative hematoxylin and eosin–stained sections from colon tissues of mice. (a) Colon tissue from mC57 mice showing absence of inflammatory cells. (b) Colon tissue of mBTBR group showing leukocyte infiltration in the mucosa and submucosa. (c) Colon tissue of fC57 group, showing absence of inflammatory cells. (d) Colon tissue from fBTBR group showing moderate leukocyte infiltration in the mucosa. Original magnification 10x. Histological evaluation of inflammatory cells infiltration was scored along the entire colon length, inspecting the colon mucosa, submucosa and transmural areas considering the following parametres: leukocyte density (mC57 = 0.33 ± 0.58, mBTBR = 2 ± 1, fC57 = 0, fBTBR = 2.67 ± 0.58) and expansion of leukocyte infiltration (mC57 = 0.33 ± 0.58, mBTBR = 1.33 ± 0.58, fC57 = 0, fBTBR = 1.67 ± 0.58). The histologic scoring system is reported in Material and Methods section. Data reported as mean ± SD, n = 3/group.

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