Figure 5: In vivo functional analysis of PlxnA1 via in utero electroporation.
From: Proteome dynamics during postnatal mouse corpus callosum development
Wild-type mouse brains electroporated at E15.5 and visualized at P15. PlxnA1 knockdown reveals more crossed callosal axons compared to the Control (control shRNA), while PlxnA1 overexpression shows a visible reduction (see arrows) [controls for overexpression (electroporation with an empty vector; data not shown) show the same results as the shRNA controls]. Cortical neuron migration and axon pathfinding appear grossly unaltered by PlxnA1 manipulation.
