Figure 1: Design of two-photon sensing platform for imaging of oxidative stress.
From: Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
(A) Illustration of the sensing mechanism. This sensing platform consists of two parts: a ruthenium complex as the turn-on fluorescent unit, and a responsive group as the GSH, H2O2 or HOCl reaction moiety. (B) Response reaction of P-GSH, P-HP and P-HA toward GSH, H2O2 or HOCl, respectively. The probes are non-fluorescent due to the effective photo-induced electron transfer process (PET). In the presence of GSH, H2O2 or HOCl, the responsive group can be quantitatively cleaved, and the reaction ruthenium complex will become highly fluorescent. The protonation state of P-HP depends on the organelle pH values. The counter-ions are sodium and potassium in biological conditions.
