Figure 2: Spectral characterization of P-GSH in vitro.
From: Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
(A) Two-photon absorption and emission spectra of P-GSH reacted with 10 μM of GSH in PBS buffer. (B) Fluorescence response of P-GSH (10 μM) to varying concentrations of GSH. (C) Emission decay of P-GSH in PBS buffer. (D) Emission decay of P-GSH in PBS buffer with GSH (20 μM). (E) FLIM images of representative P-GSH loaded AML12 cells with (lower row) or without (upper row) NEM treatment. The autofluorescence of NAD(P)H was collected at λExc/λEm: 740/350 to 450 nm. The fluorescence signal for probes and FAD was collected at λExc/λEm: 850/515 to 620 nm. Values are the mean for n = 5 replicates.
