Figure 3: Dual-mode in vivo imaging of GSH in hepatocytes of mice after APAP administration.
From: Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
(A) Representative fluorescence intensity and FLIM images of mouse liver before and 15 min after probes injection. All images were recorded at λExc/λEm: 850/515 to 620 nm. Scale bar: 20 μm. (B) Sketch to illustrate the parameters of the fit procedure of the fluorescence decay curve. Two lifetimes, τ1 and τ2 represent the fast and slow decay lifetimes. The slow decaying fluorescence accumulates on repeated laser pulsing to create enhanced background signal, defined as ‘offset’. (C) Fluorescence decay fit curves of representative area (circled in white) before (Blue) and after (Red) P-GSH injection.
