Figure 4: Dual-mode quantitative imaging of the change of oxidative stress in hepatocytes responses to NAC treatment against APAP induced liver injury.
From: Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
(A,B) Representative fluorescence intensity and FLIM images of cellular GSH or H2O2 of the control, APAP, APAP + NAC groups at 30 min after NAC treatment (75 min after APAP administration). All images were recorded at λExc/λEm: 850/515 to 620 nm. Scale bar: 20 μm. (C,E) The percentages of GSH or H2O2-positive hepatocytes of the control, APAP, APAP + NAC groups. (D,F) Population density modeling of the mean GSH or H2O2 intensity per cell in control APAP, APAP + NAC groups. Values are the mean ± s.d. for n = 5 mice; *p < 0.05, compared with APAP groups; #p < 0.05, compared with control group.
