Figure 1: PTPIP51 was increased in mouse I/R hearts.

(A) Western blot and scatter dot plots of PTPIP51 protein in mouse hearts from Sham and I/R border area. n = 4 independent experiments. α-tubulin was used as a protein loading control. *P < 0.05versus sham control group. (B) Western blot of adenoviral-mediated PTPIP51 expression (Ad-PTPIP51) in rat neonatal cardiomyocytes. Cardiomyocytes infected with adenovirus vector (Ad-lacZ) were used as a control. n = 3 independent experiments. (C) Annexin V-PI assay by flow cytometry and scatter dot plots, and (D) TUNEL staining, in Ad-lacZ and Ad-PTPIP51-infected cardiomyocytes. n = 27 (lacZ) and 20 (PTPIP51) experiments for (C), and n = 8–10 frames in each group for (D). Scale bar: 25 μm. *P < 0.05versus Ad-lacZ group. (E) PTPIP51 protein in rat neonatal cardiomyocytes infected with adenovirus-mediated PTPIP51 shRNA (sh-PTPIP51) or scrambled shRNA (sh-Scramble) as a control. n = 3 independent experiments. (F) TUNEL staining, and (G) Annexin V-PI assay, in sh-Scramble and sh-PTPIP51-infected rat neonatal cardiomyocytes. Cells were treated with 200 μM H₂O₂ for 12 h or with ddH₂O as a control. n = 10–18 frames in each group for (F) and n = 5–8 independent experiments for (G). *P < 0.05 versus scramble group; ^#P < 0.05 versus scramble group with H₂O₂ treatment.