Figure 6: PTPIP51 knockdown decreased mitochondria-SR contacts in mouse hearts.
From: PTPIP51 regulates mouse cardiac ischemia/reperfusion through mediating the mitochondria-SR junction
(A) TEM images showing mitochondria-SR ultrastructure. Orange lines are the mitochondria/SR/T-tubulecomplex. Zoomed images highlight the junctions among T-tubule (TT, green), SR (yellow), and mitochondria (Mito, Red). Scale bars: 1 μm (two left images), 500 nm (two right upper images), and 200 nm (two right bottom images). (B) Ratio of mitochondria-SR contact length/mitochondrial circumference in cardiomyocytes from sh-scramble and sh-PTPIP51 hearts. (C) Mitochondria-SR junction distance in cardiomyocytes from sh-scramble and sh-PTPIP51 hearts. (D) Numbers of mitochondria volume density in cardiomyocytes from sh-scramble and sh-PTPIP51 hearts. (E) Numbers of tethered mitochondria in 100 randomly selected mitochondria in cardiomyocytes from sh-scramble and sh-PTPIP51 hearts. n = 16, 18 frames in each group from four pairs of mouse hearts. *P < 0.05 versus scramble hearts. (F) Schematic view of PTPIP51 regulation of cardiac ischemia/reperfusion through mediating the mitochondria-SR junction.
