Figure 1: Tumour-induced DC deficiency in vitro.
From: STAT3 and NF-κB are Simultaneously Suppressed in Dendritic Cells in Lung Cancer
Human monocyte-derived DCs were generated and further incubated with or without LPS for an additional 2 days. (a) FACS assay for cell surface molecules of immature or mature DCs. Cancer serum cultured DCs are shown using a black line, and the healthy group is shown using a grey line. (b) DCs were incubated with FITC-labelled dextran. DC endocytosis was detected using flow cytometry and shown as indicated. (c) DCs induced lymphocyte proliferation. The DCs were co-cultured with PBMCs from another healthy donor at a ratio of 1:65. 3H-TdR was added on day 4, and beta liquid scintillation was measured 12–18 hours later. (d) DC cytokine secretion. Immature or mature DCs were further seeded into FCS medium without healthy or cancer sera, and the supernatant was collected and measured using BD CBA. Error bars, SEM. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001.
