Figure 3: Representative data profiles produced by Cellarium during a typical assay.
From: A platform for high-throughput bioenergy production phenotype characterization in single cells
Each curve in (A and B) represents the temporal profile of oxygen and pH sensor response, correspondingly, of one individual well of the array. (A) Oxygen sensor response. A characteristic response following the Stern-Volmer kinetics of sensor quenching by oxygen is observed in responding wells. The non-responding wells showed mostly a flat response with a slight increase in intensity during the entire experiment; (B) pH sensor response. The pH sensor intensity in responding wells decreased 2–3-fold as a result of extracellular acidification in the microwell; (C) A combined representation of OCR and ECAR calculated from the data shown in (A and B). In addition to the main cluster (blue dashed oval), several cells with high or low both OCR and ECAR are present, while the majority of cells exhibit a highly variable profile; (D) Measured cell occupancy in the wells. The cell seeding conditions were optimized to maximize the number of wells with single cell occupancy, which resulted in a significant portion of empty wells.
