Figure 2: The in vitro phenylalanine assay for ACE2 activity.
From: A Fluorometric Method of Measuring Carboxypeptidase Activities for Angiotensin II and Apelin-13
(A,B) Kinetics of enzymatic activities toward Ang II (A) and apelin-13 (B) substrates at 10 nmol per reaction with different concentrations of rsACE2. (C,D) Dose response curves of rsACE2 at situated concentration of 1 μg against Ang II (C) and apelin-13 (D) of indicated concentrations. (E,F) Kinetics of ACE2 activity measured by phenylalanine assay. The Km and Vmax for Ang II (E) and apelin-13 (F) were calculated based on Michaelis-Menten plot. Values are expressed as the mean ± S.D. from triplicate incubations.
