Figure 1: Expression pattern of ATHB17 and response to different stress signals. | Scientific Reports

Figure 1: Expression pattern of ATHB17 and response to different stress signals.

From: ATHB17 enhances stress tolerance by coordinating photosynthesis associated nuclear gene and ATSIG5 expression in response to abiotic stress

Figure 1

(A) The expression pattern of ATHB17 was revealed by GUS staining of pATHB17::GUS transgenic plants. GUS activity was observed in 2-day-old (a), 4-day-old (b), 7-day-old (c), 9-day-old (d), or 14-day-old (e) seedlings, leaves of 9-day-old plant (f), roots of mature plants (g), rosette leaf (h), cauline leaf (i), flower and young silique (j), old silique (k). (B) Analysis of the ATHB17 expression patterns by qRT–PCR. UBQ5 was used as an internal control. Values are mean ± SD of three replica experiments. (C) Expression levels of ATHB17 after different treatments. Induction levels of ATHB17 in 10-day-old plants by ABA (100 μM, 4 h), paraquat (5 μM, 4 h), drought (4 h), NaCl (200 mM, 4 h) were determined by qRT–PCR. Values are mean ± SD of three replica experiments (Student’s t-test *P < 0.05, **P < 0.01). (D) Effects of different stress treatments on pATHB17::GUS expression, 7-day-old or 14-day-old pATHB17::GUS transgenic seedlings grown on MS medium were transferred to MS liquid medium or MS liquid medium containing NaCl (200 mM), ABA (10 μM), paraquat (5 μM), mannitol (200 mM) for 1–6 h and then the seedlings were harvested for GUS staining for 3 h. (E) ATHB17 protein localization in the root cells of transgenic plants expressing ATHB17–GFP under the control of the ATHB17 native promoter.

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