Figure 3: qRT-PCR validation of the data of stress-responsive marker genes in the RNA-seq profiling.

About 0.1 g of 12-day old seedlings of the ATHB17 OX, KO lines and WT plants were treated with liquid MS medium containing 0 or 200 mM NaCl for 5 h. Total RNA were extracted and reverse-transcribed as templates for qRT-PCR. UBQ5 was used as an internal control. Values are mean ± SD of three independent experiments (*P < 0.05).