Table 2 Summary of kinetic parameters of the purified enzymes.

From: Discovery of tauroursodeoxycholic acid biotransformation enzymes from the gut microbiome of black bears using metagenomics

Enzyme

Substrate

Km (mM)

Vmax (U/mg)

kcat (S−1)

kcat/Km

S1-a-1

TCDCA

0.132

369.230

236.538

1791.954

S1-a-2

TCDCA

0.263

177.966

56.285

214.011

H1-a-1

TCDCA

0.390

70.222

10.400

26.666

H1-a-2

TCDCA

0.695

32.627

2.280

3.280

Y1-a-1

TCDCA

0.556

31.250

18.571

33.401

Clo.sa-a

TCDCA

0.241

22.470

40.103

166.402

Y1-b-1

TUDCA

6.153

65.116

12.558

2.040

Clo.sa-b

TUDCA

3.062

52.801

7.767

2.536