Figure 3: BHP kinase activity is required for stomatal opening in response to blue light. | Scientific Reports

Figure 3: BHP kinase activity is required for stomatal opening in response to blue light.

From: A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening

Figure 3

Functional complementation of stomatal opening by the introduction of the WT BHP gene (a,b) or inactive BHPD299N gene (c,d) into the bhp-1 mutant. # represents an independent transgenic line. (a,c) Expression of BHP protein in leaves of transgenic plants used for complementation experiments in (b,d). Immunoblots of BHP and H+-ATPase were performed using proteins prepared from rosette leaves of 3-week-old plants. H+-ATPase was used as a loading control. (b,d) Stomatal aperture in the transgenic lines. Data represent means ± SD (n = 4); measurement of 30 stomata in each experiment. *indicates values that statistically differ from the corresponding WT (Student’s t test; *p < 0.01).

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