Table 2 Selection of highly accurate sequence reads (10-pass CCS reads) in 12 paired samples from 6 non-SVR cases.

From: Evolution of multi-drug resistant HCV clones from pre-existing resistant-associated variants during direct-acting antiviral therapy determined by third-generation sequencing

Case #

Data size of total raw reads (FASTQ, Mb)

Data size of total 10-pass CCS read (FASTQ, Mb)

Base number of total 10-pass CCS read

Base number of selected 10-pass CCS reads*

# of selected 10-pass CCS reads

#1

Pre

1,803

7.7

3,884,777

1,176,880

375

 

Post

197

3.8

1,643,899

682,340

218

#2

Pre

1,259

17.9

8,989,951

1,539,240

508

 

Post

1,696

25.3

12,746,272

1,639,230

541

#3

Pre

955

11.1

5,518,321

598,500

285

 

Post

1,245

10.8

5,440,045

615,300

293

#4

Pre

372

3.8

1,864,548

605,775

197

 

Post

2,177

30.8

15,594,271

759,525

247

#16

Pre

2,194

41.0

21,887,194

611,716

196

 

Post

2,387

16.6

8,355,954

955,026

306

#17

Pre

2,613

35.5

17,878,934

657,090

210

 

Post

1,118

20.2

10.158,188

704,025

225

  1. CCS, circular consensus sequence; SMRT, single molecule real time; pre, pretreatment; post, at treatment failure; *, >3000 bp-long reads aligned to genotype 1b HCV genome sequence (>2000 bp-long reads in Case#3).