Figure 2: NCCM confers anabolic and anti-catabolic characteristics to degenerating NP in rat-tail injured IVD - NP. | Scientific Reports

Figure 2: NCCM confers anabolic and anti-catabolic characteristics to degenerating NP in rat-tail injured IVD - NP.

From: Molecular Therapy for Degenerative Disc Disease: Clues from Secretome Analysis of the Notochordal Cell-Rich Nucleus Pulposus

Figure 2

(A) Representative Safranin O staining sections showing fibro-cartilaginous matrix and small NP cells in rat tail injured disc NPs injected with control medium as compared to the injured disc NPs injected with serum free NCCM showing proteoglycan-rich ECM with large notochordal cells (n = 12 tissue sections/group). Representative panel showing immunohistochemistry of aggrecan, collagen 2, brachyury and Oct4 in paraffin embedded sections of rat-tail injured discs treated with protein free Hybridoma medium used as control or NCCM (Scale bar 50μ). Immunohistochemistry for all the proteins was performed in duplicates in at least 3 different IVD sections obtained from different animals from each group used in this study. (B) Representative Western blot panels showing expression of collagen 2 and the stem cell markers Oct4 and Nanog in tissue lysates obtained from healthy controls, rat-tail injured disc NPs left untreated, injected with control medium or NCCM.

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