Figure 2: Generating conditional knockout of Vilse by deletion of exon 5 (a.a. 197–648).
From: Important roles of Vilse in dendritic architecture and synaptic plasticity
(a) Schematic model for the generation of Vilse conditional knockout mice. LacZ cassette is removed by FLP recombinase, leaving exon 5 flanked by two loxP sequences. Further removal of exon 5 is conducted by Cre recombinase. (b) Camk2a-iCre-Vilsefl/+ mice were mated with Vilsefl/fl to produce VilseΔ/+ and VilseΔ/Δ littermates. Upper band indicated floxed allele cleaved by Cre recombinase and lower band indicated wild-type allele. (c) Immunohistochemistry on VilseΔ/Δ littermates showed a reduced expression of Vilse in the forebrain area. (d) Immunoblot analysis of hippocampal extracts prepared from Vilsefl/fl and VilseΔ/Δ littermates revealed the absence of Vilse in VilseΔ/Δ mice. All Western blots were processed in identical conditions and cropped from Supplementary Fig. S3c.
