Figure 1: Fat specific deletion of Hotair inhibits gluteal-femoral adipogenesis. | Scientific Reports

Figure 1: Fat specific deletion of Hotair inhibits gluteal-femoral adipogenesis.

From: Sedentary lifestyle related exosomal release of Hotair from gluteal-femoral fat promotes intestinal cell proliferation

Figure 1

(a) RT-qPCR analysis of Hotair expression in different tissues in adult mice. Relative expression levels are compared with the expression in brown fats (arbitrary value = 1) and presented as mean ± SEM, three biological replicates with three technical replicates each. (b) In situ hybridization of Hotair in gluteal fat tissue with sense (upper) and antisense (lower) probes. (c) Schematic illustration of the Hotair knockout procedure. (d,e) RT-qPCR analysis of Hotair in limb skin and gluteal fat. Relative expression levels are compared with the wildtype control (arbitrary value = 1) and presented as mean ± SEM, three biological replicates with three technical replicates each. *Indicates P < 0.05 analyzed by t test. (f–i) Gross morphology (f), brown fat (g), gluteal and femoral fat (h) and visceral fat (i) of control wildtype and fat specific Hotair KO mice. Images presented were representative of five mice. (j,k) No significant differences were found between control and cKO mice in body weight (j) and food intake (k). Data presented are representative of at least 3 mice per group.

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