Figure 4: Squeeze enhances exosomal Hotair via NFκB. | Scientific Reports

Figure 4: Squeeze enhances exosomal Hotair via NFκB.

From: Sedentary lifestyle related exosomal release of Hotair from gluteal-femoral fat promotes intestinal cell proliferation

Figure 4

(a) Immunochemistry shows nuclear translocation of NFκB p65 subunit in the gluteal fat upon squeeze. Blue nuclei staining overlapped with brown signal indicates nuclear translocation. (b) Schematic illustration of the putative NFκB binding sites in the promoter region of Hotair. (c) Chromatin Immunopreciptation (ChIP) analysis of the putative interaction between p65 and the predicted cis-elements on Hotair promoter in the squeezed adipose tissue. Relative enrichment is compared with the IgG control (arbitrary value = 1) and presented as mean ± SEM (three biological replicates with three technical replicates each), *denotes P < 0.05 by One Way ANOVA with Tukey’s post hoc test. (d,e) RT-qPCR analysis of endogenous expression of exosome biogenesis related Rab27b (d) and Alix (e) in control and squeeze treated gluteal fat tissue. Relative expression levels are compared with the control (arbitrary value = 1) and presented as mean ± SEM, n = 3, *denotes P < 0.05 by t test.

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