Figure 6: Hotair tunes Wnt pathway in a context dependent manner.

(a) Adipogenic stem cells were infected with control or Hotair overexpressing lentivirus and followed by adipogenic induction. Lipid droplets were observed by Oil O red staining. Data presented are representative of three different experiments. (b,c) RT-qPCR analysis of the expression of adipogenic marker genes Fabp4 (b) and C/ebpα (c) in cells treated same as the above. Relative expression levels are compared with the control (arbitrary value = 1) and presented as mean SEM (three biological replicates with three technical replicates each), *P < 0.05 as analyzed by t test. (d–g) Relative luciferase activity of Topflash/Fopflash reporter (d) and expression of Wnt downstream genes Cylin d1(e), Axin2 (f), and cMyc (g) in proliferating adipogenic stem cells cultured with growth medium were detected by dual luciferase assay and RT-qPCR respectively. (h–k) Relative luciferase activity of Topflash/Fopflash reporter (h) and expression of Wnt downstream genes Cylin d1 (i), Axin2 (j), and cMyc (k) in differentiating adipogenic stem cells cultured with adipogenic induction medium. Fold induction and relative expression levels are compared with the control (arbitrary value = 1) and presented as mean ± SEM (three biological replicates with three technical replicates each), *P < 0.05 as analyzed by t test.