Figure 1: Microfluidic herringbone device for capturing circulating plasma cells (CPCs) from blood samples. | Scientific Reports

Figure 1: Microfluidic herringbone device for capturing circulating plasma cells (CPCs) from blood samples.

From: Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device

Figure 1

(a) Photograph of the microfluidic chip featuring one fluidic input, one fluidic output, and 16 parallel channels for cell capture. The device is the size of a standard glass slide (25 mm × 75 mm). (b) Stacked time lapse images from a video (see Supplementary Movie S1) showing the trajectory of a fluorescently labeled plasma cell flowing in one of the microfluidic channels. The cell is shown to change its coordinates in x, y, and z (evidenced by change in focus) because of mixing induced by the herringbone geometry. (c) Schematic showing the overall concept of capture and analysis of plasma cells in the microfluidic device. Channels are coated with biotinylated CD138 antibodies (immobilized on a Neutravidin-coated surface) that capture plasma cells from the flow. Captured plasma cells are identified by staining with fluorescently labeled CD138 antibodies post capture, and intracellularly labeled with anti-κ immunoglobulin light chain antibodies following fixation. (d) Fluorescence image of captured plasma cells from a clinical sample. Red fluorescence denotes CD138, while green denotes κ light chain antibody.

Back to article page