Figure 3: Capture of plasma cells from blood. | Scientific Reports

Figure 3: Capture of plasma cells from blood.

From: Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device

Figure 3

(a) Micrographs showing capture of pre-labelled plasma cells (denoted by red arrowheads) spiked in non-diluted healthy whole blood samples introduced into the device after Ficoll fractionation or without any pre-processing with heparin or EDTA as anticoagulants. (b) Number of cells captured from 100 pre-labelled cells introduced into whole blood samples. Error bars indicate standard deviation (n = 2). (c) Fluorescence images of captured cells after in situ staining with anti-CD138 antibodies (red). Cells with both stains (top row, indicative of a spiked cell) or only CD138 stain (bottom row, indicative of plasma cells from blood denoted by white arrows) were observed.

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